Introduction

Small RNA is a kind of endogenous non coding RNA with regulatory function, mainly including miRNA, piRNA and siRNA. It induces gene silencing, participates in post transcriptional regulation, and play an important regulatory role in development, virus defense, hematopoiesis, organ formation, fine proliferation and apoptosis.

1 Small RNA transcriptome sequencing objectives

1.1 miRNA length, stem ring structure, miRNA precursor processing, and target gene binding sites and prediction

1.2 Joint analysis of miRNA-seq and mRNA-seq data

2 Bioinformatics analysis content

2.1 Sequencing quality analysis, statistics and evaluation

2.2 miRNA, rRNA, tRNA, intron, exon, snRNA, snoRNA annotation

2.3 Differential analysis of miRNA expression

2.4 Cluster analysis of miRNA expression patterns

2.5 Conservative analysis

2.6 Regulation between miRNA and target genes, and protein-protein interaction network analysis between miRNA target genes

2.7 Target gene prediction

2.8 New miRNA prediction

3 Sample requirements 

3.1 Total amount > 4 µg RNA

3.2 Concentration > 50 ng/µL