Introduction

ITRAQ technology uses a variety of isotopic reagents to label the N-terminal of protein polypeptide or lysine side chain group. After series analysis by high-precision mass spectrometer (QE or 5600), it can compare the protein expression between up to 8 samples at the same time. It has a wide range of application and can identify and quantify any type of protein, including membrane protein, nuclear protein and extracellular protein.

1 Content of bioinformatics analysis 

1.1 Raw data quality assessment 

1.2 Proteome identification 

1.3 Protein quantification 

1.4 COG, GO and KEGG pathway

1.5 Go and Pathway enrichment analysis of differential proteins 

1.6 Cluster analysis

2 Sample requirements 

2.1 Protein content > 50ug

2.2 Concentration > 5ug/uL